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Stabilization of macroscopic particle in optical trap
Mlynář, Vojtěch ; Matěj, Zdeněk (referee) ; Brablc, Martin (advisor)
Cílem této práce je implementace a otestování algoritmů pro zpětnovazební stabilizaci částice v optické pasti. Tyto algoritmy jsou implementovány v programovatelném hradlovém poli Red Pitaya STEMlab 125-14 s využitím automatického generování kódu z modelů v programu Simulink. V práci je popsán fyzikální základ řízeného systému, jeho návaznost na teorii Kálmánova filtru, detaily implementovaných algoritmů v FPGA a procedury využité pro odhad parametrů. Implementované algoritmy byly úspěšně otestovány na reálné sestavě a výsledky jsou ukázány v závěru práce.
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Correlation analysis of optically trapped particle motion
Koudelníček, Karel ; Jákl,, Petr (referee) ; Harabiš, Vratislav (advisor)
This bachelor’s thesis deals with the description of random (Brownian) motion of microscopic particle trapped by optical trap. This thesis provides overview of possible usage in biology. The major part of the thesis is dedicated to description of methods that allow characterize optical trap with etc. stiffness thanks to the random motion. First, the estimation methods are tested on the simulated data. Before using these methods for real data, it was necessary to modify them, specifically to eliminate crosstalk and frequency noise. The differences in the estimation of the stiffness from the autocorrelation function and the power spectrum were described in the conclusion.
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Noise reduction in LASER source device for optical trap
Gajzler, Vojtěch ; Adámek, Roman (referee) ; Brablc, Martin (advisor)
This work focuses on the reduction of noise in a laser source to increase the efficiency of an optical trap. The first part describes the issue of noise, methods of noise suppression, and the Red Pitaya control board. The following section thoroughly describes the program development using Simulink and Xilinx Vivado. Furthermore, the method of noise reduction using a PI controller is described. The conclusion of the work is focused on evaluating the experiment to verify the effectiveness of noise reduction.
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Stabilization of macroscopic particle in optical trap
Mlynář, Vojtěch ; Matěj, Zdeněk (referee) ; Brablc, Martin (advisor)
Cílem této práce je implementace a otestování algoritmů pro zpětnovazební stabilizaci částice v optické pasti. Tyto algoritmy jsou implementovány v programovatelném hradlovém poli Red Pitaya STEMlab 125-14 s využitím automatického generování kódu z modelů v programu Simulink. V práci je popsán fyzikální základ řízeného systému, jeho návaznost na teorii Kálmánova filtru, detaily implementovaných algoritmů v FPGA a procedury využité pro odhad parametrů. Implementované algoritmy byly úspěšně otestovány na reálné sestavě a výsledky jsou ukázány v závěru práce.
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Correlation analysis of optically trapped particle motion
Koudelníček, Karel ; Jákl,, Petr (referee) ; Harabiš, Vratislav (advisor)
This bachelor’s thesis deals with the description of random (Brownian) motion of microscopic particle trapped by optical trap. This thesis provides overview of possible usage in biology. The major part of the thesis is dedicated to description of methods that allow characterize optical trap with etc. stiffness thanks to the random motion. First, the estimation methods are tested on the simulated data. Before using these methods for real data, it was necessary to modify them, specifically to eliminate crosstalk and frequency noise. The differences in the estimation of the stiffness from the autocorrelation function and the power spectrum were described in the conclusion.
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Pokročilé techniky optických mikromanipulací
Zemánek, Pavel ; Čižmár, Tomáš ; Šiler, Martin ; Jákl, Petr ; Šerý, Mojmír ; Karásek, Vítězslav ; Brzobohatý, Oto
Nowadays the classical tool of optical micro-manipulations - optical tweezers - found numerous applications in physics, biology and chemistry. However new techniques were developed that used more sophisticated laser beam tailoring and enable to modify positions and number of manipulated objects dynamically, to sort optically objects according to their properties, to deliver them over millimetre long distances or even to let them self-organize after light illumination. We present several results dealing with the above mentioned advanced techniques developed in our laboratory.
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